The FCS-MEM tool for obtaining a distribution of diffusion times from a 1D FCS curve. To try it out you will need the LabView Run Time Engine 8.2.1 and some test data. Below is a test data file that you should be able to run with the default values. The program will automatically find optimum starting values for the Lagrange multiplier and its scaling. It will also give you the range of diffusion times that is possible with the data that has been loaded. You can also choose to use only a sub-set of the data for optimization. Please send me an email or call if you have questions.